Review



cd63 pe.cy7  (Fisher Scientific)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Fisher Scientific cd63 pe.cy7
    (A.) mRNA expression superimposed on the UMAP plot for SIGLEC6, L1CAM, and MRGPRX2. Color in the heatmap represents expression intensity with red signifying higher expression in units of z-score. (B). Gene expression of SIGLEC6, L1CAM, and MRGPRX2 in MC cluster 1 (CMA1 + ) and MC cluster 2 (CMA1 - ) trajectories across cell states along pseudotime. Each dot represents a single cell identified from clusters (MC progenitor 1, MC progenitor 2, committed MC precursor, CMA1 + and CMA1 - MC states). Color coding of each dot represents a cell’s predicted gene expressionalong the CMA1 + and CMA1 - MC developmental trajectory across pseudotime. (C). Gating strategy for identification of SIGLEC6, CD171, and MRGPRX2 expression on c-Kit + FcεRI + cells within the suspended cells released from MCFC. Live CD34 - c-Kit + FcεRI + cells (black box) were distinguished by SIGLEC6 and CD171 expression, SIGLEC6 + CD171 + (red box) and SIGLEC6 + CD171 - (blue box). Mean Fluorescence intensity level of MRGPRX2 expression was determined on SIGLEC6 + CD171 + cells (red histogram) and SIGLEC6 + CD171 - (blue histogram).. Percentage of degranulation for iPSC-derived cells as determined by β-hexosaminidase release following Antigen-dependent (IgE) (D.) and Antigen-independent activation (Substance P) (E.) Percent degranulation is calculated as (D. and E.) fraction of β-hexosaminidase activity in supernatant from stimulated cell, as compared to total hexosaminidase activity from lysed cells and (F.) Percent frequency <t>CD63</t> + CD107a + MCs (SSC high c-Kit + FcERI + ). IgE-dependent and independent degranulation assays were performed on iPSC-derived cells following 4 weeks of exposure to MC Media II. A. and C. representative of n = 3–4 independent cultures. B. Data represents mean ± SD from n = 3 independent cultures. D.- F. represents mean ± SD. Representative data from 6 experimental replicates across 4 different harvests (MM2) and 2 cell lines (D.) Representative data from 2 experimental replicates across 2 cell lines (E. & F.).
    Cd63 Pe.Cy7, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd63+pe%2Ecy7/pmc11801248-260-13-20?v=Fisher+Scientific
    Average 90 stars, based on 1 article reviews
    cd63 pe.cy7 - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "Inducible pluripotent stem cells to study human mast cell trajectories"

    Article Title: Inducible pluripotent stem cells to study human mast cell trajectories

    Journal: Mucosal immunology

    doi: 10.1016/j.mucimm.2024.07.003

    (A.) mRNA expression superimposed on the UMAP plot for SIGLEC6, L1CAM, and MRGPRX2. Color in the heatmap represents expression intensity with red signifying higher expression in units of z-score. (B). Gene expression of SIGLEC6, L1CAM, and MRGPRX2 in MC cluster 1 (CMA1 + ) and MC cluster 2 (CMA1 - ) trajectories across cell states along pseudotime. Each dot represents a single cell identified from clusters (MC progenitor 1, MC progenitor 2, committed MC precursor, CMA1 + and CMA1 - MC states). Color coding of each dot represents a cell’s predicted gene expressionalong the CMA1 + and CMA1 - MC developmental trajectory across pseudotime. (C). Gating strategy for identification of SIGLEC6, CD171, and MRGPRX2 expression on c-Kit + FcεRI + cells within the suspended cells released from MCFC. Live CD34 - c-Kit + FcεRI + cells (black box) were distinguished by SIGLEC6 and CD171 expression, SIGLEC6 + CD171 + (red box) and SIGLEC6 + CD171 - (blue box). Mean Fluorescence intensity level of MRGPRX2 expression was determined on SIGLEC6 + CD171 + cells (red histogram) and SIGLEC6 + CD171 - (blue histogram).. Percentage of degranulation for iPSC-derived cells as determined by β-hexosaminidase release following Antigen-dependent (IgE) (D.) and Antigen-independent activation (Substance P) (E.) Percent degranulation is calculated as (D. and E.) fraction of β-hexosaminidase activity in supernatant from stimulated cell, as compared to total hexosaminidase activity from lysed cells and (F.) Percent frequency CD63 + CD107a + MCs (SSC high c-Kit + FcERI + ). IgE-dependent and independent degranulation assays were performed on iPSC-derived cells following 4 weeks of exposure to MC Media II. A. and C. representative of n = 3–4 independent cultures. B. Data represents mean ± SD from n = 3 independent cultures. D.- F. represents mean ± SD. Representative data from 6 experimental replicates across 4 different harvests (MM2) and 2 cell lines (D.) Representative data from 2 experimental replicates across 2 cell lines (E. & F.).
    Figure Legend Snippet: (A.) mRNA expression superimposed on the UMAP plot for SIGLEC6, L1CAM, and MRGPRX2. Color in the heatmap represents expression intensity with red signifying higher expression in units of z-score. (B). Gene expression of SIGLEC6, L1CAM, and MRGPRX2 in MC cluster 1 (CMA1 + ) and MC cluster 2 (CMA1 - ) trajectories across cell states along pseudotime. Each dot represents a single cell identified from clusters (MC progenitor 1, MC progenitor 2, committed MC precursor, CMA1 + and CMA1 - MC states). Color coding of each dot represents a cell’s predicted gene expressionalong the CMA1 + and CMA1 - MC developmental trajectory across pseudotime. (C). Gating strategy for identification of SIGLEC6, CD171, and MRGPRX2 expression on c-Kit + FcεRI + cells within the suspended cells released from MCFC. Live CD34 - c-Kit + FcεRI + cells (black box) were distinguished by SIGLEC6 and CD171 expression, SIGLEC6 + CD171 + (red box) and SIGLEC6 + CD171 - (blue box). Mean Fluorescence intensity level of MRGPRX2 expression was determined on SIGLEC6 + CD171 + cells (red histogram) and SIGLEC6 + CD171 - (blue histogram).. Percentage of degranulation for iPSC-derived cells as determined by β-hexosaminidase release following Antigen-dependent (IgE) (D.) and Antigen-independent activation (Substance P) (E.) Percent degranulation is calculated as (D. and E.) fraction of β-hexosaminidase activity in supernatant from stimulated cell, as compared to total hexosaminidase activity from lysed cells and (F.) Percent frequency CD63 + CD107a + MCs (SSC high c-Kit + FcERI + ). IgE-dependent and independent degranulation assays were performed on iPSC-derived cells following 4 weeks of exposure to MC Media II. A. and C. representative of n = 3–4 independent cultures. B. Data represents mean ± SD from n = 3 independent cultures. D.- F. represents mean ± SD. Representative data from 6 experimental replicates across 4 different harvests (MM2) and 2 cell lines (D.) Representative data from 2 experimental replicates across 2 cell lines (E. & F.).

    Techniques Used: Expressing, Fluorescence, Derivative Assay, Activation Assay, Activity Assay



    Similar Products

    cd63 pe.cy7  (Fisher Scientific)
    90
    Fisher Scientific cd63 pe.cy7
    (A.) mRNA expression superimposed on the UMAP plot for SIGLEC6, L1CAM, and MRGPRX2. Color in the heatmap represents expression intensity with red signifying higher expression in units of z-score. (B). Gene expression of SIGLEC6, L1CAM, and MRGPRX2 in MC cluster 1 (CMA1 + ) and MC cluster 2 (CMA1 - ) trajectories across cell states along pseudotime. Each dot represents a single cell identified from clusters (MC progenitor 1, MC progenitor 2, committed MC precursor, CMA1 + and CMA1 - MC states). Color coding of each dot represents a cell’s predicted gene expressionalong the CMA1 + and CMA1 - MC developmental trajectory across pseudotime. (C). Gating strategy for identification of SIGLEC6, CD171, and MRGPRX2 expression on c-Kit + FcεRI + cells within the suspended cells released from MCFC. Live CD34 - c-Kit + FcεRI + cells (black box) were distinguished by SIGLEC6 and CD171 expression, SIGLEC6 + CD171 + (red box) and SIGLEC6 + CD171 - (blue box). Mean Fluorescence intensity level of MRGPRX2 expression was determined on SIGLEC6 + CD171 + cells (red histogram) and SIGLEC6 + CD171 - (blue histogram).. Percentage of degranulation for iPSC-derived cells as determined by β-hexosaminidase release following Antigen-dependent (IgE) (D.) and Antigen-independent activation (Substance P) (E.) Percent degranulation is calculated as (D. and E.) fraction of β-hexosaminidase activity in supernatant from stimulated cell, as compared to total hexosaminidase activity from lysed cells and (F.) Percent frequency <t>CD63</t> + CD107a + MCs (SSC high c-Kit + FcERI + ). IgE-dependent and independent degranulation assays were performed on iPSC-derived cells following 4 weeks of exposure to MC Media II. A. and C. representative of n = 3–4 independent cultures. B. Data represents mean ± SD from n = 3 independent cultures. D.- F. represents mean ± SD. Representative data from 6 experimental replicates across 4 different harvests (MM2) and 2 cell lines (D.) Representative data from 2 experimental replicates across 2 cell lines (E. & F.).
    Cd63 Pe.Cy7, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd63+pe%2Ecy7/pmc11801248-260-13-20?v=Fisher+Scientific
    Average 90 stars, based on 1 article reviews
    cd63 pe.cy7 - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A.) mRNA expression superimposed on the UMAP plot for SIGLEC6, L1CAM, and MRGPRX2. Color in the heatmap represents expression intensity with red signifying higher expression in units of z-score. (B). Gene expression of SIGLEC6, L1CAM, and MRGPRX2 in MC cluster 1 (CMA1 + ) and MC cluster 2 (CMA1 - ) trajectories across cell states along pseudotime. Each dot represents a single cell identified from clusters (MC progenitor 1, MC progenitor 2, committed MC precursor, CMA1 + and CMA1 - MC states). Color coding of each dot represents a cell’s predicted gene expressionalong the CMA1 + and CMA1 - MC developmental trajectory across pseudotime. (C). Gating strategy for identification of SIGLEC6, CD171, and MRGPRX2 expression on c-Kit + FcεRI + cells within the suspended cells released from MCFC. Live CD34 - c-Kit + FcεRI + cells (black box) were distinguished by SIGLEC6 and CD171 expression, SIGLEC6 + CD171 + (red box) and SIGLEC6 + CD171 - (blue box). Mean Fluorescence intensity level of MRGPRX2 expression was determined on SIGLEC6 + CD171 + cells (red histogram) and SIGLEC6 + CD171 - (blue histogram).. Percentage of degranulation for iPSC-derived cells as determined by β-hexosaminidase release following Antigen-dependent (IgE) (D.) and Antigen-independent activation (Substance P) (E.) Percent degranulation is calculated as (D. and E.) fraction of β-hexosaminidase activity in supernatant from stimulated cell, as compared to total hexosaminidase activity from lysed cells and (F.) Percent frequency CD63 + CD107a + MCs (SSC high c-Kit + FcERI + ). IgE-dependent and independent degranulation assays were performed on iPSC-derived cells following 4 weeks of exposure to MC Media II. A. and C. representative of n = 3–4 independent cultures. B. Data represents mean ± SD from n = 3 independent cultures. D.- F. represents mean ± SD. Representative data from 6 experimental replicates across 4 different harvests (MM2) and 2 cell lines (D.) Representative data from 2 experimental replicates across 2 cell lines (E. & F.).

    Journal: Mucosal immunology

    Article Title: Inducible pluripotent stem cells to study human mast cell trajectories

    doi: 10.1016/j.mucimm.2024.07.003

    Figure Lengend Snippet: (A.) mRNA expression superimposed on the UMAP plot for SIGLEC6, L1CAM, and MRGPRX2. Color in the heatmap represents expression intensity with red signifying higher expression in units of z-score. (B). Gene expression of SIGLEC6, L1CAM, and MRGPRX2 in MC cluster 1 (CMA1 + ) and MC cluster 2 (CMA1 - ) trajectories across cell states along pseudotime. Each dot represents a single cell identified from clusters (MC progenitor 1, MC progenitor 2, committed MC precursor, CMA1 + and CMA1 - MC states). Color coding of each dot represents a cell’s predicted gene expressionalong the CMA1 + and CMA1 - MC developmental trajectory across pseudotime. (C). Gating strategy for identification of SIGLEC6, CD171, and MRGPRX2 expression on c-Kit + FcεRI + cells within the suspended cells released from MCFC. Live CD34 - c-Kit + FcεRI + cells (black box) were distinguished by SIGLEC6 and CD171 expression, SIGLEC6 + CD171 + (red box) and SIGLEC6 + CD171 - (blue box). Mean Fluorescence intensity level of MRGPRX2 expression was determined on SIGLEC6 + CD171 + cells (red histogram) and SIGLEC6 + CD171 - (blue histogram).. Percentage of degranulation for iPSC-derived cells as determined by β-hexosaminidase release following Antigen-dependent (IgE) (D.) and Antigen-independent activation (Substance P) (E.) Percent degranulation is calculated as (D. and E.) fraction of β-hexosaminidase activity in supernatant from stimulated cell, as compared to total hexosaminidase activity from lysed cells and (F.) Percent frequency CD63 + CD107a + MCs (SSC high c-Kit + FcERI + ). IgE-dependent and independent degranulation assays were performed on iPSC-derived cells following 4 weeks of exposure to MC Media II. A. and C. representative of n = 3–4 independent cultures. B. Data represents mean ± SD from n = 3 independent cultures. D.- F. represents mean ± SD. Representative data from 6 experimental replicates across 4 different harvests (MM2) and 2 cell lines (D.) Representative data from 2 experimental replicates across 2 cell lines (E. & F.).

    Article Snippet: Cells were stained with CD117 A488 (clone 104D2, Mouse IgG1, kappa, Fisher Scientific), CD63 Pe.Cy7 (clone H5C6, Mouse IgG1, kappa, Fisher Scientific), CD107a APC (clone H4A3, Mouse IgG1, kappa, Fisher Scientific), PE conjugated anti-FcεRI (clone AER-37, Mouse IgG2b, kappa, Fisher Scientific), Fixable Live/Dead PB in FACS buffer at 4 °C for 20 min.

    Techniques: Expressing, Fluorescence, Derivative Assay, Activation Assay, Activity Assay